Nardoo Hills supports more than 110 bird species, including the nationally endangered Swift Parrot, which travels all the way from Tasmania during winter to feed on flowering eucalypts. It also protects:
Hooded robin (threatened in Victoria), Diamond firetail (threatened in Victoria), Lace monitor (endangered in Victoria), Fat-tailed dunnart, Chocolate wattled bat, Brown treecreeper, Crested bellbird (threatened in Victoria), Crested shrike-tit.
Yellow box, Drooping sheoak, Creamy candles, Buloke, Northern golden moths orchid (threatened in Victoria), Southern swainson pea, Robust greenhood orchid (nationally critically endangered).
Plains grassy woodland (endangered), Hillcrest herb-rich woodland, Metamorphic slopes shrubby woodland, Box-ironbark forest, Broombush mallee.